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total rna  (TaKaRa)


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    Structured Review

    TaKaRa total rna
    Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+rna/TaKaRa+MiniBEST+Universal+RNA+Extraction+Kit/pm42822105-129-10-24
    Average 97 stars, based on 4330 article reviews
    total rna - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Cyclometalated iridium complex with red-light activation for dual ferroptosis-apoptosis cancer therapy.
    Article Snippet: Total RNA was extracted from the cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions, and RNA concentration and purity were assessed by the A260/A280 ratio using a NanoDrop spectrophotometer. .. An equal amount of total RNA (1 μg) was reverse-transcribed into cDNA using the PrimeScript RT Reagent Kit (TaKaRa, Kusatsu, Japan; cat. no. RR037A). .. Quantitative PCR was performed with Hieff qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China; cat. no. 11201ES08) on a real-time PCR instrument model using the following primers: GPX4 forward, 5′-GCCTGGATAAGTACAGGGGTT-3′, and reverse, 5′-CATGCAGATCGACTAGCTGAG-3’; GAPDH forward, 5′- TGGATTTGGACGCATTGGTC-3′, and reverse, 5′-TTTGCACTGGTACGTGTTGAT-3’.

    Article Title: IGF2BP1 regulates chicken skeletal muscle development through an m6A-dependent inter-organ regulatory axis.
    Article Snippet: A NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was employed to evaluate RNA concentration and purity, while agarose gel electrophoresis confirmed integrity. .. Total RNA (1 μg) was reverse-transcribed into cDNA with the PrimeScript RT Reagent Kit containing gDNA Eraser (TaKaRa, Dalian, China). .. Amplification reactions were subsequently conducted on a QuantStudio 5 platform using TB Green Premix Ex Taq II (TaKaRa).

    Article Title: Inhibition of CDK9 improves colonic epithelial dysfunction in DSS-induced ulcerative colitis mice by blocking the IL-6/STAT3 signaling pathway.
    Article Snippet: Background: Ulcerative colitis (UC) is driven by relentless mucosal inflammation and epithelial barrier compromise.. Cyclin-dependent kinase 9 (CDK9) governs transcriptional elongation, yet its therapeutic potential in restoring the intestinal barrier via the STAT3 signaling axis remains underexplored.. Methods: The effects of the selective CDK9 inhibitor, LDC000067 (LDC), in dextran sulfate sodium (DSS)-induced colitis mice and lipopolysaccharide (LPS)-stimulated Caco-2 cells were investigated.

    Article Title: Epitranscriptomic stabilization of PGRMC1 by the METTL3-IGF2BP1 axis promotes ovarian cancer progression.
    Article Snippet: Ovarian cancer (OV) remains one of the most lethal gynecological malignancies, with limited effective early detection strategies and therapeutic options.. Despite advances in platinum-based chemotherapy and targeted therapies, including PARP inhibitors and immune checkpoint inhibitors, treatment failure and recurrence remain common.. Here, we integrated scRNA-seq data from OV tumours (GSE154600) with copy-number variation analysis and malignant transcriptional metaprogram analysis, and weighted gene co-expression network analysis to identify glycolysis-associated hub genes.

    Article Title: INHBB Promotes Colorectal Cancer Progression by Inducing CD8 + T Cell Exhaustion via the ACVR2B ‐ SMAD Signaling Axis
    Article Snippet: RNA quality and concentration were quantified by NanoDrop spectrophotometer. .. Equal amounts of total RNA (1 μg) were reverse‐transcribed into cDNA using the PrimeScript RT reagent Kit (Takara). .. qPCR amplification was carried out on a CFX96 real‐time PCR detection system (Bio‐Rad) with SYBR Green qPCR Master Mix (Takara).

    Synthesized:

    Article Title: M2 macrophage exosomes reverse heart failure post-myocardial infarction by suppressing type 1 interferon signaling in myeloid cells.
    Article Snippet: Effective treatment strategies to alleviate heart failure that develops as a consequence of myocardial infarction (MI) remain an unmet need in cardiovascular medicine.. In this study, we uncover that exosomes produced by human Tohoku Hospital Pediatrics-1 (THP-1) macrophages cultured with the cytokine interleukin-4 (THP1-IL4-exo) reverse cardiac functional decline in mice that developed MI in response to diet-induced occlusive coronary atherosclerosis.. The therapeutic benefits of THP1-IL4-exo stem from their ability to drive transcriptional reprogramming of inflammatory responses in myeloid cells.

    RNA Sequencing:

    Article Title: M2 macrophage exosomes reverse heart failure post-myocardial infarction by suppressing type 1 interferon signaling in myeloid cells.
    Article Snippet: Effective treatment strategies to alleviate heart failure that develops as a consequence of myocardial infarction (MI) remain an unmet need in cardiovascular medicine.. In this study, we uncover that exosomes produced by human Tohoku Hospital Pediatrics-1 (THP-1) macrophages cultured with the cytokine interleukin-4 (THP1-IL4-exo) reverse cardiac functional decline in mice that developed MI in response to diet-induced occlusive coronary atherosclerosis.. The therapeutic benefits of THP1-IL4-exo stem from their ability to drive transcriptional reprogramming of inflammatory responses in myeloid cells.

    Control:

    Article Title: Effects of cordycepin on antioxidant capacity and proliferative potential of buffalo spermatogonial cells in vitro.
    Article Snippet: Buffalo spermatogonial cells (bSCs) are essential for maintaining spermatogenesis and male fertility.. Oxidative stress impairs bSCs proliferation through ROS-induced damage and apoptosis.. Cordycepin, a bioactive compound from Cordyceps militaris, exhibits potent antioxidant effects but has not been systematically evaluated in bSCs.

    RNA Extraction:

    Article Title: Effects of cordycepin on antioxidant capacity and proliferative potential of buffalo spermatogonial cells in vitro.
    Article Snippet: Buffalo spermatogonial cells (bSCs) are essential for maintaining spermatogenesis and male fertility.. Oxidative stress impairs bSCs proliferation through ROS-induced damage and apoptosis.. Cordycepin, a bioactive compound from Cordyceps militaris, exhibits potent antioxidant effects but has not been systematically evaluated in bSCs.



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